Abstract. Tetranychus evansi Baker & Pritchard, 1960 (Tetranychidae) is a phytophagous mite with wide distribution and high importance for agriculture. This study reports the occurrence of T. evansi in Nicotiana alata Link & Otto, Nicotiana forgetiana Sander ex W. Watson (Solanaceae) and their putative hybrids in a greenhouse in Rio Grande do Sul state, Brazil. Different stages of development of T. evansi were found in N. alata and N. forgetiana and in their putative hybrids.