Resumo. Zabrotes subfasciatus (Boheman, 1833) is a cosmopolitan pest, occurring in all countries that grow dry bean, Phaseolus vulgaris L. To assure the development of quality bioassays with this insect, a rearing procedure was implemented according to Standards of Good Laboratory Practices. An average of 2,751.94 adults was produced from 300 couples, in a kilogram of dry bean, in approximately one month. The rearing methodology described herein has been conducted successfully for several generations, providing insects of quality, which have been used routinely for academic and research purposes.