Resumo. BACKGROUND: Stretching exercises increase the joint range of motion (ROM) and depend on the skeletal tissues' exposition-time to stretch. However, it is unclear how a long stretching time affects the muscle-tendon unit's passive mechanical properties. AIM: This study aimed to analyze changes in the triceps surae muscle-tendon unit’s passive mechanical properties before and after a 10-minute passive stretching protocol. METHOD: Thirty healthy participants (26.57 ± 3.82 years old) were allocated into a control group (n=15), who did not perform any intervention, and to an experimental group (n=15), who performed one bout of a 10-minute ankle plantar flexor passive static stretching. Ankle ROM, plantar flexor passive torque, and myotendinous junction displacement were evaluated pre-intervention, immediately after, and 15, 30, 45, and 60 minutes after the end of the intervention. The stiffnesses of the muscle-tendon unit, muscle, and tendon were calculated for all moments. A generalized estimating equation test was performed to compare groups and moments. RESULTS: The experimental group increased the ROM (p<0.001) from pre- to post-intervention and remained augmented up to 60 minutes. The myotendinous junction displacement decreased at post-30 and post-45 moments compared to pre-intervention. Muscular stiffness increased immediately after stretching and post-45 and post-60 minutes. Passive torque and musculotendinous unit stiffness decreased over time, with trivial, small, and moderate effect sizes, respectively. CONCLUSION: Passive static stretching (10 min) generates an acute ROM increase associated with muscle-tendon unit passive mechanical properties reduction, which lasts up to one-hour post-intervention.