Resumo. Collections carried out for a period of 10 weeks from October to December 2013 in two fragments of Cerrado (experimental farm of Embrapa Gado de Corte and Private Reserve of Natural Heritage belong to the Universidade Federal de Mato Grosso do Sul (RPPN-UFMS)) located in the municipality of Campo Grande, state of Mato Grosso do Sul, Midwestern Brazil, with traps baited with decomposing beef liver, and collections conducted for a period of 15 days in January 2014 in the RPPN-UFMS, using Shannon traps baited with dog corpses, resulted in 32 flesh fly species of eight genera, with the first record of the genus Blaesoxipha and 15 new species records to Mato Grosso do Sul.